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It makes use of a porous stationary stage which allows scaled-down molecules to enter the pores, causing extended retention periods. Size exclusion HPLC is usually used from the Evaluation of polymers, proteins, along with other macromolecules. It is derived from column chromatography with enhanc

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A little volume of sample to be analyzed is introduced on the mobile period stream and is also retarded by certain chemical or Actual physical interactions While using the stationary phase. 5% drinking water coupled with a better proportion of a significantly less polar solvent. HILIC is popularl